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The sections were blocked with normal goat serum and then incubated with anti-MUC2, anti-Nrf2 (1:200) at 4 overnight, followed by biotinylated secondary antibodies

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Chemical shifts for the plant-extracted compounds were assigned using 1D 1 H and 13 C and 2D COSY, HSQC, and HMBC nuclear magnetic resonance (NMR) experiments and compared to the 1 H chemical shifts of the enzyme assay-produced compounds

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This process enables horses to access stored fat more efficiently, which supports endurance during exercise and helps prevent fatigue