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Euthanasia was performed by carbon dioxide (CO 2 ) inhalation, following ethical guidelines to minimize distress

Lastly, utilizing RNAScope in situ hybridization in the lower intestines of mice, we observed that GLP-1 cells expressed generic markers of secretory cells such as Snap25 and Nefm , but neither synaptic markers such as Syn1 and Nrxn2 , nor glutamatergic markers such as Slc17a7

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The intensive phase involves daily manual lymphatic drainage performed by certified lymphedema therapists (CLT), multilayer compression bandaging, remedial exercises, and meticulous skin care

J Androl 2012;33(4):615-623