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This work is supported by ERC grant (SPARK 101140863)

You and I both have very personal experiences that have shaded and driven our desire to bring gut health

Reverse transcription (RT) of mRNA to cDNA was performed with Superscript II Reverse Transcriptase (Invitrogen) and Oligo(dT) 18 primers (ThermoScientific) after DNA removal with RNase-free DNase I (Invitrogen)

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Getting the B12 Shot Before getting the shot, make sure to inform your doctor of any allergies, nutritional supplements you may be taking, or if youve recently consumed large amounts of alcohol

The peptide vial research guide covers proper handling in more detail if you want additional context on best practices