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Sirtuin inhibition attenuates the production of inflammatory cytokines in lipopolysaccharide-stimulated macrophages

doi: 10.1016/j.jprot.2017.05.003 153 ViswanathanV

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The GATEWAY transformation system (Thermo Fisher Scientific) was used to convert the inserted fragment into an n-terminal 3xFLAG, His, HA, GST, or Myc labeled destination vector (for expression in mammalian cells)

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